Platform for Imaging by Microscopy

Technologies and microscope features offered at the PIM:

1.  Confocal microscopy, laser scanning and spinning disk – high contrast and resolution, optical sectioning
   • SP8, SP8-DLS, SP8-STED, SP8-MP, SD-TIRF, Stellaris WLL+
2. Spectral imaging – free choice of emission wavelength range without filters
   • SP8, SP8-DLS, SP8-STED, SP8-MP, Stellaris WLL+
3. White light laser – free choice of excitation wavelength between 470 and 670 nm
   • SP8-STED (470-670 nm), Stellaris WLL+ (440-790 nm)
4. Multi-photon microscopy – powerful laser of long wavelength (infrared) to penetrate deep into tissue
   • SP8-MP
5. Super-resolution microscopy – acquisition (and image processing) methods to go beyond limit of resolution in fluorescence microscopy
   • STED nanoscopy: SP8-STED
   • “Lightning” deconvolution-based super-resolution: SP8, SP8-DLS, SP8-MP, Stellaris WLL+
6. Total Internal Reflection Microscopy (TIRF) – imaging a very thin layer of fluorescence
   • SD-TIRF
7. FRAP (Fluorescence Recovery after Photobleaching) – molecular dynamics of defined sample regions by targeted bleaching; mechanism can also be used for guided photo-activation etc.
   • SP8, SP8-DLS, SP8-STED, SP8-MP, SD-TIRF, Stellaris WLL+
8. Light-sheet imaging – fast, gentle fluorescence imaging of larger volume samples (several mm3)
   • SP8-DLS
9. Separation of a wide range of near-infrared fluorophores
   • Stellaris WLL+
10. TauSense, 
    • Stellaris WLL+
11. SPECTRAPLEX, for spatial biology
    • Stellaris WLL+
12. Live cell imaging – environmental control (temperature, CO2, humidity) for long-term imaging of cells, organoids, embryos
    • SD-TIRF, SP8-STED, SP8-DLS, (SP8), DMi8-1, DMi8-2, DMi8-3, Stereo, Stellaris WLL+
13. Rapid imaging – mechanisms to speed up time-lapse or other multi-dimensional image capture projects
    • Resonant scanning (8000 Hz): SP8, SP8-STED, SP8-MP, Stellaris WLL+
    • Fast camera capture: SD-TIRF, DMi8-1, DMi8-3, SP8-DLS (en mode DLS), AxioScan, Stellaris WLL+
14. Automatic sample scanning – extending the field of view by capturing a mosaic of overlapping images and stitching them into a composite 
    • Fully automatic: AxioScan
    • Semi-automatic: SD-TIRF, SP8, SP8-STED, SP8-DLS, Stellaris WLL+, SP8-MP, DMi8-1, DMi8-2, DMi8-3
15. Long working distance objectives – imaging up to several millimeters into samples at intermediate to high resolution
    • DMi8-1, DMi8-3, SP8-MP, SP8-DLS
16. Transmitted light and fluorescence detection
    • Light: SD-TIRF, SP8, SP8-STED, SP8-DLS, Stellaris WLL+, DMi8-1, DMi8-2, DMi8-3, AxioScan, stereo (également la lumière réfléchie)
    • Fluorescence: SD-TIRF, SP8, SP8-STED, SP8-DLS, Stellaris WLL+, DMi8-1, DMi8-2, DMi8-3, AxioScan, stereo
17. Monochrome and color detection – fast, light-sensitive cameras and detectors
    • Color: AxioScan, DMi8-1, DMi8-3, stereo
    • Monochrome: SD-TIRF, SP8, SP8-STED, SP8-DLS, Stellaris WLL+, SP8-MP, DMi8-1, DMi8-2, DMi8-3, AxioScan, stereo
18. Large samples – imaging samples of 1 mm or more in diameter in a single field of view
    • Macroscopy: Stereo
19. Upright and inverted configurations – objectives above or below the sample for working with different sample types
    • Upright: SP8-MP, AxioScan, Stereo, SP8-DLS
    • Inverted: SD-TIRF, SP8, SP8-STED, SP8-DLS, Stellaris WLL+, DMi8-1, DMi8-2, DMi8-3

Platform Manager
Elke Kuester-Schoeck 
514 345-4931, extension 5231
elke.kuster.hsj@ssss.gouv.qc.ca

Microscopy specialist
Vanesa Jimenez Amilburu
514-345-4931, ext.5231
vanesa.jimenez.amilburu.hsj@ssss.gouv.qc.ca

Co-directors
Gilles Hickson
Graziella Di Cristo

Head, Scientific Platforms
Valérie Villeneuve
514 345-4931, extension 2786
valerie.villeneuve.hsj@ssss.gouv.qc.ca

Please fill this online form for any usage request
We will then contact you to arrange for a short consultation to discuss your imaging needs and set up training as needed.

If you are already a user of the platform portal, you can request training in the “my profile” section.

• Booking portal
  Registration required by contacting the Platform Manager

• CHU Sainte-Justine Foundation
• Canada Foundation for Innovation 
• Leica Microsystems