Technologies offered
Technologies and microscope features offered at the PIM :
- Confocal microscopy, laser scanning and spinning disk – high contrast and resolution, optical sectioning
- SP8, SP8-DLS, SP8-STED, SP8-MP, SD-TIRF
- Spectral imaging – free choice of emission wavelength range without filters
- SP8, SP8-DLS, SP8-STED, SP8-MP
- White light laser – free choice of excitation wavelength between 470 and 670 nm
- Multi-photon microscopy – powerful laser of long wavelength (infrared) to penetrate deep into tissue
- Super-resolution microscopy – acquisition (and image processing) methods to go beyond limit of resolution in fluorescence microscopy
- STED nanoscopy : SP8-STED
- “Lightning” deconvolution-based super-resolution: SP8, SP8-DLS, SP8-MP
- Total Internal Reflection Microscopy (TIRF) – imaging a very thin layer of fluorescence
- FRAP (Fluorescence Recovery after Photobleaching) – molecular dynamics of defined sample regions by targeted bleaching; mechanism can also be used for guided photo-activation etc.
- SP8, SP8-DLS, SP8-STED, SP8-MP, SD-TIRF
- Light-sheet imaging – fast, gentle fluorescence imaging of larger volume samples (several mm3)
- Laser Micro-Dissection (LMD) – precise cutting of defined regions of microscopic specimen
- Live cell imaging – environmental control (temperature, CO2, humidity) for long-term imaging of cells, organoids, embryos
- SD-TIRF, SP8-STED, SP8-DLS, (SP8), DMi8-1, DMi8-2, Stereo
- Rapid imaging – mechanisms to speed up time-lapse or other multi-dimensional image capture projects
- Resonant scanning (8000 Hz): SP8, SP8-STED, SP8-MP
- Fast camera capture : SD-TIRF, DMi8-1, DMi8-2, SP8-DLS (in DLS mode), AxioScan
- Automatic sample scanning – extending the field of view by capturing a mosaic of overlapping images and stitching them into a composite
- Fully automatic with slide feeder: AxioScan
- Semi-automatic : SD-TIRF, SP8, SP8-STED, SP8-DLS, SP8-MP, DMi8-1, DMi8-2, DMi8-3, DM6-1
- Long working distance objectives – imaging up to several millimeters into samples at intermediate to high resolution
- DMi8-1, DMi8-3, SP8-MP, SP8-DLS
- Monochrome and color detection – fast, light-sensitive cameras and detectors
- Color : AxioScan, DM6-2, DMi8-3, stereo, LMD
- Monochrome : SD-TIRF, SP8, SP8-STED, SP8-DLS, SP8-MP, DMi8-1, DMi8-2, DMi8-3, DM6-1, AxioScan, stereo
- Transmitted light and fluorescence detection
- Transmitted light : SD-TIRF, SP8, SP8-STED, SP8-DLS, DMi8-1, DMi8-2, DMi8-3, DM6-1, DM6-2, AxioScan, stereo (also reflected light)
- Fluorescence : SD-TIRF, SP8, SP8-STED, SP8-DLS, DMi8-1, DMi8-2, DMi8-3, DM6-1, AxioScan, stereo
- Large samples – imaging samples of 1 mm or more in diameter in a single field of view
- Upright and inverted configurations – objectives above or below the sample for working with different sample types
- Upright : SP8-MP, DM6-1, DM6-2, AxioScan, Stereo, LMD, SP8-DLS
- Inverted : SD-TIRF, SP8, SP8-STED, SP8-DLS, DMi8-1, DMi8-2, DMi8-3
Which technology can be done on which instrument?
| |
SD-TIRF |
SP8 |
SP8-DLS |
SP8-STED |
SP8-MP |
LMD |
DM6-1 |
DM6-2 |
DMi8-1 |
DMi8-2 |
DMi8-3 m |
AxioScan |
Stereo |
| Confocal |
SD |
LS |
LS |
LS |
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| Spectral |
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+ |
+ |
+ |
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| White light laser |
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+ |
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| Multi-photon |
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+ |
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| TIRF |
+ |
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| Super-resolution |
|
(+) |
(+) |
+ |
(+) |
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| FRAP |
+ |
+ |
+ |
+ |
+ |
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| Light-sheet imaging |
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+ |
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| Laser micro-dissection |
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+ |
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| Live cell imaging |
+ |
(+) |
+ |
+ |
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+ |
+ |
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+ |
| Rapid imaging |
+ |
+ |
+ (DLS) |
+ |
+ |
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+ |
+ |
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+ |
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| Automatic sample scanning (mosaic) |
(+) |
(+) |
(+) |
(+) |
(+) |
(+) |
(+) |
(+) |
(+) |
(+) |
(+) |
+ |
|
| Long working distance objectives |
|
|
(+) |
|
+ |
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+ |
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+ |
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+ |
| Detection (monochr./colour) |
M |
M |
M |
M |
M |
C, M |
M |
C |
M |
M |
C, M |
C, M |
C, M |
| Fluorescence |
+ |
+ |
+ |
+ |
+ |
+ |
+ |
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+ |
+ |
+ |
+ |
+ |
| Large samples |
|
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(+) |
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(+) |
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+ |
| Stand |
inv |
inv |
inv/upr |
inv |
upr |
upr |
upr |
upr |
inv |
inv |
inv |
upr |
upr |
Team
Usage request and rules
Online booking portal
- Booking portal
Registration required by contacting the Platform Manager
Financial partners
- CHU Sainte-Justine Foundation
- Canada Foundation for Innovation
- Leica Microsystems