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Platform for Imaging by Microscopy

DMi8 inverted microscopes: Leica DMi8 inverted wide-field fluorescence microscopes

Three wide-field microscopes (Leica DMi8) on an inverted stand. Motorized stage for precise sample movement. Fast, sensitive monochrome and/or color cameras. Fluorescence illumination (Leica EL6000 metal-halide lamp). User-changeable excitation/emission filter cubes. One system with a fast external excitation filter wheel for high-speed fluorescence filter changes. Environmental control for temperature, CO2, humidity, available on two systems via a stage-top incubator (Tokai Hit). Micro-manipulator/-injection on one system.


DMi8-1 (fast filter-wheel):

5x HC PL FLUOTAR 5x/0.15
10x HC PL FL 10x/0.30
20x HC PL FL L 20x/0.40 CORR
40x HC PL FLUOTAR L 40x/0.60
63x/oil HC PL APO 63x/1.40-0.60 OIL


5x HC PL FLUOTAR 5x/0.15
10x HC PL FLUOTAR 10x/0.30
20x HC PL APO 20x/0.80
40x HC PL APO 40x/0.85 CORR
63x/oil HC PL APO 63x/1.40-0.60 OIL
100x/oil HCX PL APO 100x/1.40 OIL

DMi8-3 (micro-manipulator):

2.5x HC PL FLUOTAR 2.5x/0.07
5x HC PL FLUOTAR 5x/0.15
10x HC PL FLUOTAR 10x/0.32
20x HC PL FLUOTAR L 20x/0.40 CORR
40x HC PL FLUOTAR L 40x/0.60 CORR
63x/oil HC PL APO 63x/1.40-0.60 OIL

Fluorescence filter sets:

Name Color (emission) Excitation Dichroic Emission Only on ...
DAPI Blue BP 350/50 400 BP 460/50  
GFP Green BP 470/40 500 BP 525/50  
RHOD Orange-Red (Rhodamine) BP 546/10 560 BP 585/40  
Y3 Orange-Red (Cy3) BP 545/30 565 BP 610/75  
TXR Deep-red (Texas Red) BP 560/40 585 BP 630/75  
Y5 Far-red BP 620/60 660 BP 700/75
D/F/TX-P Triple : Blue, Green, Deep-red EFW* : 402/15, 490/20, 572/35 435, 505, 595 455/25, 525/35, 630/60 DMi8-1
FURA2 Ca-imaging EFW* : 340/26, 380/11 400 510/80 DMi8-1

* EFW = External filter wheel, only available on DMi8-1

About this page
Updated on 8/3/2022
Created on 12/1/2017
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