Three wide-field microscopes (Leica DMi8) on an inverted stand. Motorized stage for precise sample movement. Fast, sensitive monochrome and/or color cameras. Fluorescence illumination (Leica EL6000 metal-halide lamp). User-changeable excitation/emission filter cubes. One system with a fast external excitation filter wheel for high-speed fluorescence filter changes. Environmental control for temperature, CO2, humidity, available on two systems via a stage-top incubator (Tokai Hit). Micro-manipulator/-injection on one system.
Objectives:
DMi8-1 (fast filter-wheel):
| 5x |
HC PL FLUOTAR 5x/0.15 |
| 10x |
HC PL FL 10x/0.30 |
| 20x |
HC PL FL L 20x/0.40 CORR |
| 40x |
HC PL FLUOTAR L 40x/0.60 |
| 63x/oil |
HC PL APO 63x/1.40-0.60 OIL |
DMi8-2:
| 5x |
HC PL FLUOTAR 5x/0.15 |
| 10x |
HC PL FLUOTAR 10x/0.30 |
| 20x |
HC PL APO 20x/0.80 |
| 40x |
HC PL APO 40x/0.85 CORR |
| 63x/oil |
HC PL APO 63x/1.40-0.60 OIL |
| 100x/oil |
HCX PL APO 100x/1.40 OIL |
DMi8-3 (micro-manipulator):
| 2.5x |
HC PL FLUOTAR 2.5x/0.07 |
| 5x |
HC PL FLUOTAR 5x/0.15 |
| 10x |
HC PL FLUOTAR 10x/0.32 |
| 20x |
HC PL FLUOTAR L 20x/0.40 CORR |
| 40x |
HC PL FLUOTAR L 40x/0.60 CORR |
| 63x/oil |
HC PL APO 63x/1.40-0.60 OIL |
Fluorescence filter sets:
| Name |
Color (emission) |
Excitation |
Dichroic |
Emission |
Only on ... |
| DAPI |
Blue |
BP 350/50 |
400 |
BP 460/50 |
|
| GFP |
Green |
BP 470/40 |
500 |
BP 525/50 |
|
| RHOD |
Orange-Red (Rhodamine) |
BP 546/10 |
560 |
BP 585/40 |
|
| Y3 |
Orange-Red (Cy3) |
BP 545/30 |
565 |
BP 610/75 |
|
| TXR |
Deep-red (Texas Red) |
BP 560/40 |
585 |
BP 630/75 |
|
| Y5 |
Far-red |
BP 620/60 |
660 |
BP 700/75 |
| D/F/TX-P |
Triple : Blue, Green, Deep-red |
EFW* : 402/15, 490/20, 572/35 |
435, 505, 595 |
455/25, 525/35, 630/60 |
DMi8-1 |
| FURA2 |
Ca-imaging |
EFW* : 340/26, 380/11 |
400 |
510/80 |
DMi8-1 |
* EFW = External filter wheel, only available on DMi8-1