Inverted fluorescence microscope on a Leica DMi8 stand offering spinning disk confocal microscopy (Yokogawa CSU X-1 confocal head with Yokogawa filter wheel), TIRF light path (VisiTIRF), and FRAP technology (VisiFRAP). Detection with sensitive and fast cameras (Hamamatsu ImageEM X2 EM-CCD for SD confocal, Orca Flash 4.0 sCMOS for TIRF or confocal). Automatic focus stabilization (Leica AFC). Environmental control for live cell imaging via a temperature-controlled box around the microscope and a stage-top incubator for CO2 and humidity control.
Objectives:
10x |
HC PL APO 10x/0.45 |
20x |
HC PL APO 20x/0.80 |
40x |
N PL L 40x/0.55 CORR |
63x/oil |
HC PL APO 63x/1.47 OIL CORR TIRF |
100x/oil |
HC PL APO 100x/1.47 OIL CORR TIRF |
Lasers for confocal and TIRF excitation:
405 nm |
for blue emitters |
445 nm |
for cyan emitters (only SD confocal lightpath) |
488 nm |
for green emitters |
561 nm |
for green emitters |
640 nm |
for far-red emitters |