Laser scanning confocal microscope (Leica TCS SP8) on an inverted stand, fully motorized stage, fast z movement (Leica Super Z Galvo stage). Five internal detectors (three HyD high-sensitivity, two PMT) with spectral detection, one transmitted light detector (PMT). DLS light sheet microscopy module for rapid imaging of larger samples. Environmental control for temperature, CO2, humidity, possible through a mobile stage-top incubator (Tokai Hit).
Objectives for confocal imaging:
| 5x |
HC PL FLUOTAR 5x/0.15 |
| 10x |
HC PL APO 10x/0.40 CS2 |
| 20x |
HC PL APO 20x/0.75 CS2 |
| 40x |
HCX PL APO 40x/0.85 CORR CS,0.11- |
| 63x/oil |
HC PL APO 63x/1.40 OIL CS2 |
Objectives for light sheet imaging:
| 5x DSL |
HC PL FLUOTAR 5X/0.15 IMM DLS |
| 10x DSL |
HC APO L 10x/0.30 W DLS |
| 25x DSL |
HC FLUOTAR L 25x/0.95 W DLS |
Lasers for fluorescence excitation:
| 405 nm |
for blue emitters |
| 488 nm |
for green emitters |
| 552 nm |
for red emitters |
| 638 nm |
for far-red emitters |