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Platform for Imaging by Microscopy

SP8-STED: Leica TCS SP8 laser scanning confocal microscope with STED super-resolution

Laser scanning confocal microscope (Leica TCS SP8) on an inverted stand, fully motorized stage, fast z movement (Leica Super Z Galvo stage). Resonant scanner (8 Hz) for fast scan times and lower photo-toxicity or bleaching. White light laser allows free choice of excitation wavelength between 470 and 670 nm. STED (stimulated emission-depletion) super-resolution through two depletion lasers, 592 and 775 nm. Five internal detectors (two HyD high-sensitivity with time gating, three PMT) with spectral detection, one transmitted light detector (PMT). Environmental control of temperature, CO2, humidity, possible through a mobile stage-top incubator (Tokai Hit).


10x HC PL APO 10x/0.40 CS2
20x/multi-imm HC PL APO 20x/0.75IMM CORR CS2
40x/oil HCX PL APO 40x/1.30 Oil CS2
63x/oil HC PL APO 63x/1.40 OIL CS2
93x/glycerol mot HC PL APO 93x/1.30 GLUC motC STED W
100x/oil STED HC PL APO 100x/1.40 CS2

Lasers for fluorescence excitation:

405 nm for blue emitters
442 nm for cyan emitters
470-670 nm pulsed white light laser for fluorophores from blue-green to far-red emission

Lasers for STED depletion:

592 nm continuous wave (non-pulsed) laser
775 nm pulsed laser
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Updated on 12/1/2017
Created on 12/1/2017
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