Laser scanning confocal microscope with spectral emission detection (Leica TCS SP8) on an inverted stand, fully motorized stage, fast z movement (Leica Super Z Galvo stage). Resonant scanner (8 Hz) for fast scan times and lower photo-toxicity or bleaching. White light laser allows free choice of excitation wavelength between 470 and 670 nm. STED (stimulated emission-depletion) super-resolution through two depletion lasers, 592 and 775 nm. Five internal detectors (two HyD high-sensitivity with time gating, three PMT), one transmitted light detector (PMT). Environmental control of temperature, CO2, humidity, possible through a mobile stage-top incubator (Tokai Hit).
Objectives:
| 10x |
HC PL APO 10x/0.40 CS2 |
| 20x/multi-imm |
HC PL APO 20x/0.75IMM CORR CS2 |
| 40x/oil |
HCX PL APO 40x/1.30 Oil CS2 |
| 63x/oil |
HC PL APO 63x/1.40 OIL CS2 |
| 93x/glycerol mot |
HC PL APO 93x/1.30 GLUC motC STED W |
| 100x/oil STED |
HC PL APO 100x/1.40 CS2 |
Lasers for fluorescence excitation:
| 405 nm |
for blue emitters |
| 442 nm |
for cyan emitters |
| 470-670 nm |
pulsed white light laser for fluorophores from blue-green to far-red emission |
Lasers for STED depletion:
| 592 nm |
continuous wave (non-pulsed) laser |
| 775 nm |
pulsed laser |