Laser scanning confocal microscope with spectral emission detection (Leica TCS SP8) on an upright stand, motorized stage that can accommodate large samples (Scientifica). Resonant scanner (8 Hz) for fast scan times and lower photo-toxicity or bleaching. Pulsed infrared laser for multi-photon excitation with two lines: tuneable 700-1300 nm, and fixed 1040 nm (INSight DS+ DUAL). Four external, non-descanned detectors (two HyD high-sensitivity, two PMT), three internal detectors (one HyD high-sensitivity, two PMT), one transmitted light detector (PMT). Full enclosure to protect from stray light interference.
Objectives:
| 10x |
HC PL FLUOTAR 10x/0.3 |
| 25x/water mot |
HC IRAPO L 25x/1.00 W motCORR |
| 25x/multi mot |
HC FLUOTAR L 25x/1.00 IMM motCORR VISIR |
| 40x/water |
HCX APO L 40x/0.80 W UVI |
| 63x/glycerol |
HC PL APO 63x/1.30 GLYC CORR CS2 |
Lasers for fluorescence excitation:
| 405 nm |
for blue emitters |
| 488 nm |
for green emitters |
| 552 nm |
for red emitters |
| 638 nm |
for far-red emitters |
| 1040 nm, 700-1300 nm |
for multi-photon excitation |