Laser scanning confocal microscope (Leica TCS SP8) on an upright stand, motorized stage that can accommodate large samples (Scientifica). Resonant scanner (8 Hz) for fast scan times and lower photo-toxicity or bleaching. Pulsed infrared laser for multi-photon excitation with two lines: tuneable 700-1300 nm, and fixed 1040 nm (INSight DS+ DUAL). Four external, non-descanned detectors (two HyD high-sensitivity, two PMT), three internal detectors (one HyD high-sensitivity, two PMT) with spectral detection, one transmitted light detector (PMT). Full enclosure to protect from stray light interference.
Objectives:
| 10x |
HC PL FLUOTAR 10x/0.3 |
| 25x/water mot |
HC IRAPO L 25x/1.00 W motCORR |
| 25x/multi mot |
HC FLUOTAR L 25x/1.00 IMM motCORR VISIR |
| 40x/water |
HCX APO L 40x/0.80 W UVI |
| 63x/glycerol |
HC PL APO 63x/1.30 GLYC CORR CS2 |
Lasers for fluorescence excitation:
| 405 nm |
for blue emitters |
| 488 nm |
for green emitters |
| 552 nm |
for red emitters |
| 638 nm |
for far-red emitters |
| 1040 nm, 700-1300 nm |
for multi-photon excitation |