• Français

Platform for Imaging by Microscopy

DMi8 inverted microscopes: Leica DMi8 inverted wide-field fluorescence microscopes

Three wide-field microscopes (Leica DMi8) on an inverted stand. Motorized stage for precise sample movement. Fast, sensitive monochrome and/or color cameras. Fluorescence illumination (Leica EL6000 metal-halide lamp). User-changeable excitation/emission filter cubes. One system with a fast external excitation filter wheel for high-speed fluorescence filter changes. Environmental control for temperature, CO2, humidity, available on two systems via a stage-top incubator (Tokai Hit). Micro-manipulator/-injection on one system.

Objectives:

DMi8-1 (fast filter-wheel):

5x HC PL FLUOTAR 5x/0.15
10x HC PL FL 10x/0.30
20x HC PL FL L 20x/0.40 CORR
40x HC PL FLUOTAR L 40x/0.60
63x/oil HC PL APO 63x/1.40-0.60 OIL

DMi8-2:

5x HC PL FLUOTAR 5x/0.15
10x HC PL FLUOTAR 10x/0.30
20x HC PL APO 20x/0.80
40x HC PL APO 40x/0.85 CORR
63x/oil HC PL APO 63x/1.40-0.60 OIL
100x/oil HCX PL APO 100x/1.40 OIL

DMi8-3 (micro-manipulator):

2.5x HC PL FLUOTAR 2.5x/0.07
5x HC PL FLUOTAR 5x/0.15
10x HC PL FLUOTAR 10x/0.32
20x HC PL FLUOTAR L 20x/0.40 CORR
40x HC PL FLUOTAR L 40x/0.60 CORR
63x/oil HC PL APO 63x/1.40-0.60 OIL

Fluorescence filter sets:

Name Color (emission) Excitation Dichroic Emission Only on ...
DAPI Blue BP 350/50 400 BP 460/50  
GFP Green BP 470/40 500 BP 525/50  
RHOD Orange-Red (Rhodamine) BP 546/10 560 BP 585/40  
Y3 Orange-Red (Cy3) BP 545/30 565 BP 610/75  
TXR Deep-red (Texas Red) BP 560/40 585 BP 630/75  
Y5 Far-red BP 620/60 660 BP 700/75
D/F/TX-P Triple : Blue, Green, Deep-red EFW* : 402/15, 490/20, 572/35 435, 505, 595 455/25, 525/35, 630/60 DMi8-1
FURA2 Ca-imaging EFW* : 340/26, 380/11 400 510/80 DMi8-1

* EFW = External filter wheel, only available on DMi8-1

About this page
Updated on 8/3/2022
Created on 12/1/2017
Alert or send a suggestion
 

Grow Beyond our wildest dreams

With the support of donors like you, at the heart of the Grow Beyond campaign, we are leading healthcare teams towards the opportunities offered by science and new technologies, so that every child, no matter where in Quebec, has access to the unique expertise and know-how of CHU Sainte-Justine. Together, let's join forces for their future.

Grow Beyond with us.

Contact Us

514 345-4931

Légal

© 2006-2014 CHU Sainte-Justine.
All rights reserved.
Terms of Use, Confidentiality, Security

Avertissement

Les informations contenues dans le site « CHU Sainte-Justine » ne doivent pas être utilisées comme un substitut aux conseils d’un médecin dûment qualifié et autorisé ou d’un autre professionnel de la santé. Les informations fournies ici le sont à des fins exclusivement éducatives et informatives.

Consultez votre médecin si vous croyez être malade ou composez le 911 pour toute urgence médicale.

CHU Sainte-Justine