Platform for Imaging by Microscopy

SD-TIRF: Leica/Quorum VisiScope 4 Elements

Inverted fluorescence microscope on a Leica DMi8 stand offering spinning disk confocal microscopy (Yokogawa CSU X-1 confocal head with Yokogawa filter wheel), TIRF light path (VisiTIRF), and FRAP technology (VisiFRAP). Detection with sensitive and fast cameras (Hamamatsu ImageEM X2 EM-CCD for SD confocal, Orca Flash 4.0 sCMOS for TIRF or confocal). Automatic focus stabilization (Leica AFC). Environmental control for live cell imaging via a temperature-controlled box around the microscope and a stage-top incubator for CO2 and humidity control.

Objectives:

10x HC PL APO 10x/0.45
20x HC PL APO 20x/0.80
40x N PL L 40x/0.55 CORR
63x/oil HC PL APO 63x/1.47 OIL CORR TIRF
100x/oil HC PL APO 100x/1.47 OIL CORR TIRF

Lasers for confocal and TIRF excitation:

405 nm for blue emitters
445 nm for cyan emitters (only SD confocal lightpath)
488 nm for green emitters
561 nm for green emitters
640 nm for far-red emitters
About this page
Updated on 12/1/2017
Created on 12/1/2017
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