Platform for Imaging by Microscopy

SP8-STED: Leica TCS SP8 laser scanning confocal microscope with STED super-resolution

Laser scanning confocal microscope (Leica TCS SP8) on an inverted stand, fully motorized stage, fast z movement (Leica Super Z Galvo stage). Resonant scanner (8 Hz) for fast scan times and lower photo-toxicity or bleaching. White light laser allows free choice of excitation wavelength between 470 and 670 nm. STED (stimulated emission-depletion) super-resolution through two depletion lasers, 592 and 775 nm. Five internal detectors (two HyD high-sensitivity with time gating, three PMT) with spectral detection, one transmitted light detector (PMT). Environmental control of temperature, CO2, humidity, possible through a mobile stage-top incubator (Tokai Hit).

Objectives:

10x HC PL APO 10x/0.40 CS2
20x/multi-imm HC PL APO 20x/0.75IMM CORR CS2
40x/oil HCX PL APO 40x/1.30 Oil CS2
63x/oil HC PL APO 63x/1.40 OIL CS2
93x/glycerol mot HC PL APO 93x/1.30 GLUC motC STED W
100x/oil STED HC PL APO 100x/1.40 CS2

Lasers for fluorescence excitation:

405 nm for blue emitters
442 nm for cyan emitters
470-670 nm pulsed white light laser for fluorophores from blue-green to far-red emission

Lasers for STED depletion:

592 nm continuous wave (non-pulsed) laser
775 nm pulsed laser
About this page
Updated on 12/1/2017
Created on 12/1/2017
Alert or send a suggestion

Every dollar counts!

Thank you for your generosity.

It's people like you that allow us to accelerate research and heal more children better every year and, as such, offer among the best healthcare in the world.

It's also possible to give by mail or by calling toll-free

1-888-235-DONS (3667)

Contact Us

514 345-4931

Légal

© 2006-2014 CHU Sainte-Justine.
All rights reserved.
Terms of Use, Confidentiality, Security

Avertissement

Les informations contenues dans le site « CHU Sainte-Justine » ne doivent pas être utilisées comme un substitut aux conseils d’un médecin dûment qualifié et autorisé ou d’un autre professionnel de la santé. Les informations fournies ici le sont à des fins exclusivement éducatives et informatives.

Consultez votre médecin si vous croyez être malade ou composez le 911 pour toute urgence médicale.

CHU Sainte-Justine