Platform for Imaging by Microscopy

SP8 multi-photon: Leica TCS SP8 MP laser scanning confocal microscope with multi-photon illumination

Laser scanning confocal microscope (Leica TCS SP8) on an upright stand, motorized stage that can accommodate large samples (Scientifica). Resonant scanner (8 Hz) for fast scan times and lower photo-toxicity or bleaching. Pulsed infrared laser for multi-photon excitation with two lines: tuneable 700-1300 nm, and fixed 1040 nm (INSight DS+ DUAL). Four external, non-descanned detectors (two HyD high-sensitivity, two PMT), three internal detectors (one HyD high-sensitivity, two PMT) with spectral detection, one transmitted light detector (PMT). Full enclosure to protect from stray light interference.

Objectives:

10x HC PL FLUOTAR 10x/0.3
25x/water mot HC IRAPO L 25x/1.00 W motCORR
25x/multi mot HC FLUOTAR L 25x/1.00 IMM motCORR VISIR
40x/water HCX APO L 40x/0.80 W UVI
63x/glycerol HC PL APO 63x/1.30 GLYC CORR CS2

Lasers for fluorescence excitation:

405 nm for blue emitters
488 nm for green emitters
552 nm for red emitters
638 nm for far-red emitters
1040 nm, 700-1300 nm for multi-photon excitation


 

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Updated on 12/1/2017
Created on 12/1/2017
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