Researcher

    Sonia Cellot , M.D. , Ph.D.

    sonia.cellot@umontreal.ca
    Sonia Cellot
    Research Axis
    Viral and Immune Disorders and Cancers
    Research Theme
    Cancer: genetic and molecular mechanisms, and new therapies
    Address
    CHUSJ - Centre de Recherche

    Phone
    514 345-4931 #6026

    Title

    • Hematologist Pediatrician, CHU Sainte-Justine
    • Clinical Assistant Professor, Department of Pediatrics, Université de Montréal

    Research Interests

    • Hematopoietic stem cells (HSC)
    • Chromatin methylation
    • Pediatric acute myeloid leukemia (AML)

    Long-lived hematopoietic stem cells (HSC) sustain the constant production of all mature cell lineages in the blood system. HSC constitute the critical cellular component of transplantation procedures performed in oncology for high risk malignancies, including leukemia. Nonetheless, factors that dictate HSC fate remain poorly defined, and their isolation and ex vivo culture remain challenging, limiting their clinical applications.

    Projects conducted as part of her graduate studies aimed at developing more efficient HSC expansion strategies in vitro. Then, optimisation of screening assays to identify HSC regulators using functional genetics was pursued. Multiple gene candidates could thus be tested simultaneously, using a pipeline of standardized HSC isolation, transduction and transplantation strategies, through overexpression and knockdown studies. Several HSC regulators were identified using these strategies. The main focus of the laboratory is to study the role of chromatin methylation in human HSC biology. Accessibility of the transcriptional machinery to the genetic code is mediated through the concerted actions of chromatin modifying enzymes. The Mll (Mixed Lineage Leukemia) gene, encoding for a histone methyltransferase (of lysine 4 on histone 3, or H3K4) is involved in normal HSC maintenance and mutated in >70% of infant leukemia cases. Once thought to be irreversible, histone methylation is now regarded as a highly dynamic process, since the identification of the histone demethylase LSD1 (KDM1A) in 2004. Previous work identified that JARID1B (KDM5B), an eraser of the H3K4 epigenetic mark, regulates the expression of stemness associated genes, and its knockdown leads to HSC expansion in culture (Cellot S., Hope K.J. et al, Blood 2013). The implication of histone demethylases (HDM) in HSC biology and cancer development is being unraveled, and under active pharmacological scrutiny.

    Career Summary

    Dr. Sonia Cellot is an assistant professor in the Department of Pediatrics at Université de Montréal and an investigator in the Viral and Immune Disorders and Cancers axis at CHU Sainte-Justine Research Center., She works as a pediatric hematologist in the Hematology-Oncology Division of CHU Sainte-Justine since 2009, where she is responsible for the pediatric acute myeloid leukemia (AML) program. Clinical duties encompass direct patient care, mainly in the HSC transplantation unit, and diagnostic test development as medical advisor in the molecular biology laboratory.

    Dr. Cellot is co-investigator with Dr. Josée Hébert of the pediatric branch of the Quebec Leukemia Cell Bank, an initiative aiming to systematically preserve pediatric AML samples for research purposes. She holds a PhD degree in molecular biology from Université de Montréal, where she worked on the identification of nuclear factors that regulate HSC activity. Her research focused on the epigenetic regulators of human HSC and pediatric AML.

    Fundings

    • CHU Sainte-Justine Foundation
    • Cole Foundation
    • Fondation Centre de cancérologie Charles-Bruneau
    • Fonds de recherche du Québec – Santé (FRQS)
    • Terry Fox Research Institute

    Presentations

    • Cellot S., Hope K.J., Sauvageau M., Chagraoui J., Deneault, É. Mayotte N., MacRae T., Wilhelm B.T, Sauvageau, G. A functional in vivo RNAi screen involving JmjC domain containing candidates unravels Jarid1b as a negative modulator of hematopoietic stem cell self-renewal. American Society of Hematology (ASH) meeting, San Diego, Dec. 2011.
    • Pécheux L., Teira P., Bittencourt H., Laramée L., Dormoy-Raclet V., Duval M., Hébert J., Haddad E., Cellot S. GATA2 mutations screening is recommended prior to related transplant for selected cases of pediatric myelodysplastic syndrome (MDS). Canadian Blood and Marow Transplantation Group (GBMTG) annual conferes. Winnipeg, 2013.

Publications

Predictors and outcomes of viridans group streptococcal infections in pediatric acute myeloid leukemia: from the Canadian infections in AML research group - Lewis V, Yanofsky R, Mitchell D, Dix D, Ethier MC, Gillmeister B, Johnson D, Michon B, Stobart K, Portwine C, Silva M, Cellot S, Price V, Bowes L, Zelcer S, Brossard J, Beyene J, Sung L. Predictors and outcomes of viridans group streptococcal infections in pediatric acute myeloid leukemia: from the Canadian infections in AML research group. Pediatr Infect Dis J 2014; 33(2): 126-129.

Hepatitis E virus seroprevalence before hematopoietic SCT: a pediatric experience - Jaber M, Béland K, Rousseau C, Cellot S, Halac U, Alvarez F, Buteau C. Hepatitis E virus seroprevalence before hematopoietic SCT: a pediatric experience. Bone Marrow Transplant 2014; (sous presse).

RNAi screen identifies Jarid1b as a major regulator of mouse HSC activity - Cellot S, Hope K, Chagraoui J, Sauvageau M, Deneault E, Macrae T, Mayotte N, Wilhelm BT, Landry J, Ting SB, Krosl J, Humphries K, Thompson A, Sauvageau G. RNAi screen identifies Jarid1b as a major regulator of mouse HSC activity. Blood 2013; 122(9): 1545-1555.

Entinostat prevents leukemia maintenance in a collaborating oncogene-dependent model of cytogenetically normal acute myeloid leukemia - Ramsey J, Kettyle LM, Sharpe DJ, Mulgrew NM, Dickson GJ, Bijl JJ, Austin P, Mayotte N, Cellot S, Krosl J, Sauvageau G, Thompson A, Lappin TR, Zhang SD, Mills KI, Krosl J, Sauvageau G, Thompson A. Entinostat prevents leukemia maintenance in a collaborating oncogene-dependent model of cytogenetically normal acute myeloid leukemia. Stem Cells 2013; 31(7): 1434-1445.

Infections in pediatric acute promyelocytic leukemia: from the canadian infections in acute myeloid leukemia research group - Cellot S, Johnson D, Dix D, Ethier MC, Gillmeister B, Mitchell D, Yanofsky R, Lewis V, Portwine C, Price V, Zelcer S, Silva M, Bowes L, Michon B, Stobart K, Brossard J, Beyene J, Sung L. Infections in pediatric acute promyelocytic leukemia: from the canadian infections in acute myeloid leukemia research group. BMC Cancer 2013; 13(1): 276.

Infectious events prior to chemotherapy initiation in children with acute myeloid leukemia - Portwine C, Mitchell D, Johnson D, Gillmeister B, Ethier MC, Yanofsky R, Dix D, Cellot S, Lewis V, Price V, Silva M, Zelcer S, Bowes L, Michon B, Stobart K, Brossard J, Beyene J, Sung L. Infectious events prior to chemotherapy initiation in children with acute myeloid leukemia. PLoS ONE 2013; 8(4): e61899.

Clostridium difficile infection in pediatric acute myeloid leukemia: from the Canadian Infections in Acute Myeloid Leukemia Research Group - Price V, Portwine C, Zelcer S, Ethier MC, Gillmeister B, Silva M, Schindera C, Yanofsky R, Mitchell D, Johnston D, Lewis V, Dix D, Cellot S, Michon B, Bowes L, Stobart K, Brossard J, Beyene J, Sung L. Clostridium difficile infection in pediatric acute myeloid leukemia: from the Canadian Infections in Acute Myeloid Leukemia Research Group. Pediatr Infect Dis J 2013; 32(6): 610-613.

Invasive fungal infections in paediatric acute myeloid leukaemia - Johnston D, Lewis V, Yanofsky R, Gillmeister B, Ethier MC, Mitchell D, Cellot S, Dix D, Portwine C, Price V, Silva M, Zelcer S, Michon B, Bowes L, Stobart K, Brossard J, Beyene J, Sung L. Invasive fungal infections in paediatric acute myeloid leukaemia. Mycoses 2013; 56(4): 482-487.

Infections in children with down syndrome and acute myeloid leukemia: a report from the Canadian infections in AML research group - Tran T, Mitchell D, Dix D, Cellot S, Ethier MC, Gillmeister B, Hitzler J, Lewis V, Yanofsky R, Johnston D, Portwine C, Price V, Zelcer S, Silva M, Michon B, Bowes L, Stobart K, Brossard J, Beyene J, Sung L. Infections in children with down syndrome and acute myeloid leukemia: a report from the Canadian infections in AML research group. Infect Agent Cancer 2013; 8(1): 47.

Association between corticosteroids and infection, sepsis, and infectious death in pediatric acute myeloid leukemia: results from the Canadian infections in AML research group - Dix D, Cellot S, Price V, Gillmeister B, Ethier MC, Johnston D, Lewis V, Michon B, Mitchell D, Stobart K, Yanofsky R, Portwine C, Silva M, Bowes L, Zelcer S, Brossard J, Traubuci J, Allen U, Beyene J, Sung L. Association between corticosteroids and infection, sepsis, and infectious death in pediatric acute myeloid leukemia: results from the Canadian infections in AML research group. Clin Infect Dis 2012; 55(12): 1608-1614.

Asymmetric segregation and self-renewal of hematopoietic stem and progenitor cells with endocytic Ap2a2 - Ting SB, Deneault E, Hope K, Cellot S, Chagraoui J, Mayotte N, Dorn JF, Laverdure JP, Harvey M, Hawkins ED, Russell SM, Maddox PS, Iscove NN, Sauvageau G. Asymmetric segregation and self-renewal of hematopoietic stem and progenitor cells with endocytic Ap2a2. Blood 2012; 119(11): 2510-2522.

A role for GPx3 in activity of normal and leukemia stem cells - Herault O, Hope K, Deneault E, Mayotte N, Chagraoui J, Wilhelm BT, Cellot S, Sauvageau M, Andrade-Navarro MA, Hébert J, Sauvageau G. A role for GPx3 in activity of normal and leukemia stem cells. J Exp Med 2012; 209(5): 895-901.

An RNAi screen identifies Msi2 and Prox1 as having opposite roles in the regulation of hematopoietic stem cell activity - Hope K, Cellot S, Ting SB, Macrae T, Mayotte N, Iscove NN, Sauvageau G. An RNAi screen identifies Msi2 and Prox1 as having opposite roles in the regulation of hematopoietic stem cell activity. Stem Cells 2010; 7(1): 101-113.

A functional screen to identify novel effectors of hematopoietic stem cell activity - Deneault E, Cellot S, Faubert A, Laverdure JP, Fréchette M, Chagraoui J, Mayotte N, Sauvageau M, Ting SB, Sauvageau G. A functional screen to identify novel effectors of hematopoietic stem cell activity. Cell 2009; 137(2): 369-379.

Sustained in vitro trigger of self-renewal divisions in Hoxb4hiPbx1(10) hematopoietic stem cells - Cellot S, Krosl J, Chagraoui J, Meloche S, Humphries RK, Sauvageau G. Sustained in vitro trigger of self-renewal divisions in Hoxb4hiPbx1(10) hematopoietic stem cells. Exp Hematol 2007; 35(5): 802-816.

About this page
Edited by Hoffmann Maude
Updated on 7/2/2015
Created on 9/18/2014
Alert or send a suggestion

Every dollar counts!

Thank you for your generosity.

It's people like you that allow us to accelerate research and heal more children better every year and, as such, offer among the best healthcare in the world.

It's also possible to give by mail or by calling toll-free

1-888-235-DONS (3667)

Contact Us

514 345-4931

Légal

© 2006-2014 CHU Sainte-Justine.
All rights reserved.
Terms of Use, Confidentiality, Security

Avertissement

Les informations contenues dans le site « CHU Sainte-Justine » ne doivent pas être utilisées comme un substitut aux conseils d’un médecin dûment qualifié et autorisé ou d’un autre professionnel de la santé. Les informations fournies ici le sont à des fins exclusivement éducatives et informatives.

Consultez votre médecin si vous croyez être malade ou composez le 911 pour toute urgence médicale.

CHU Sainte-Justine